![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4790086:Design
Designed by: Hongyan Jing Group: iGEM23_NNU-CHINA (2023-10-11)
ASR6(node 112)/pET-22b(+)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 442
Illegal PstI site found at 453
Illegal PstI site found at 579 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 429
Illegal NheI site found at 960
Illegal PstI site found at 442
Illegal PstI site found at 453
Illegal PstI site found at 579 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 449
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 442
Illegal PstI site found at 453
Illegal PstI site found at 579 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 442
Illegal PstI site found at 453
Illegal PstI site found at 579
Illegal NgoMIV site found at 143
Illegal NgoMIV site found at 425 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
None.
Source
The original sequence of pET-22b was downloaded from NCBI. Node 112 was constructed through Ancestral Sequence Reconstruction (ASR).